Fig. 6
Effect of BCCAO with/without Aβ1-40 on neuronal degeneration. a Neuronal degeneration in the hippocampus 3 weeks after injection evaluated by histological analysis with HE staining. Representative photographs in each group were from the same location in the DG area, × 400 magnification. Neurons with rich cytoplasm and round and slightly stained nucleus with relatively large and clear nucleolus formation indicate normal cells (blue arrow). Neurons shrunken with condensed and deeply stained nucleus indicate pyknotic cells (red arrow). Quantification of total neuronal cell number and percentage of pyknotic neurons calculated and analyzed at × 400 are shown. n = 5 for each group in WT and smAT2, respectively. b Expressions of apoptosis genes in the hippocampus 3 weeks after injection by real-time RT-PCR. n = 6 for each group in WT and smAT2, respectively. *P < 0.05, †P < 0.01 vs. PBS, BCCAO (−), respectively