Fig. 4

Effects of JWH-133 pretreatment on the perivascular expression of TLR4 and MMP9 after I/R injury in the gray matter of spinal cord. a Representative immunofluorescence images of CB2R (green), TLR4 (green), and MMP9 (green) colocalized with capillary endothelial cells (CD31; red) in the Sham and I/R groups at day 2 after injury. Arrows show the perivascular expression of CB2R, TLR4, and MMP9. Scale bars are 75 μm. b Higher magnification images of TLR4 (green) and MMP9 (green) colocalized with capillary endothelial cells (CD31; red) in each group at day 2 after injury. Scale bars are 30 μm. c Quantification of CB2R, TLR4, and MMP9 is represented as the average IOD. CB2R, TLR4, and MMP9 positive fluorescence labeling significantly increased in I/R group contrasting to that in sham group. d Quantification of TLR4/MMP9 perivascular expression is represented as the average IOD ratio of perivascular TLR4/MMP9 to corresponding CD31. I/R injury increased TLR4/MMP9 perivascular expression compared to sham group. The positive fluorescence labeling of TLR4/MMP9 along microvessels were obviously lower in JWH or TAK group compared to I/R group. All results are represented as mean ± SEM (n = 5). *P < 0.05 vs. sham group; #P < 0.05 vs. I/R group