Fig. 6

Effects of JWH-133 pretreatment on the colocalization of CB2R, TLR4, and MMP9 with reactive astrocytes after I/R injury in the gray matter of spinal cord. a Representative immunofluorescence images of CB2R (green), TLR4 (green), and MMP9 (green) colocalized with reactive astrocytes (GFAP; red) at day 2 after injury. The arrows show the colocalization fluorescence labeling (yellow). Scale bars are 30 μm. b Quantification of the colocalization expression for target proteins with reactive astrocytes is represented as the average IOD of the colocalization fluorescence labeling. The astrocytic TLR4/MMP9 in JWH group was significantly less than that in I/R group. All results are represented as mean ± SEM (n = 5). #P < 0.05 vs. I/R group