Fig. 10

TLR2, but not caspase-1, influences early intracellular survival in microglia and macrophages. Gentamicin protection assays were performed on primary microglia or bone marrow-derived macrophages from WT, TLR2 KO, and caspase-1 (Casp-1) KO mice using a MOI of 10:1 (bacteria to cell). Time course of intracellular bacterial survival in (a) microglia and (b) macrophages with (c) quantification of intracellular burdens at time 0 (immediately following high dose gentamicin treatment). Results are from one experiment (n = 4 biological replicates/treatment) that was independently replicated four times and analyzed by one-way ANOVA with Tukey’s multiple comparison test (**p < 0.01)