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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Mouse connective tissue mast cell proteases tryptase and carboxypeptidase A3 play protective roles in itch induced by endothelin-1

Fig. 2

The mast cell protease-deficient mouse lines Mcpt4-/-Mcpt6-/-Cpa3-/-, Cpa3Y356L,E378A, and Mcpt6-/- scratch more than wild-type controls when injected with ET-1. a When injected with ET-1 (20 pmol), Mcpt4-/-Mcpt6-/-Cpa3-/- mice (n = 14) had a much higher number of scratching episodes in 60 min than wild-type controls (WT, n = 12). b Mcpt4-/-Mcpt6-/-Cpa3-/- mice spent more total time scratching during the 60 min test. c The Mcpt4-/-Mcpt6-/-Cpa3-/- mice also had shorter scratching episodes on average than controls. d The results from (a) analyzed over time. The Mcpt4-/-Mcpt6-/-Cpa3-/- mice scratched more frequently than controls overall (vertical significance bar), and post hoc analysis showed that they scratched significantly more in the first four intervals of the test. e Both Mcpt6-/- (n = 8) and Cpa3Y356L,E378A mice (Cpa3Y3, n = 8) scratched more frequently in 60 min than wild-type controls (n = 11) when injected with ET-1. Mcpt4-/- mice (n = 7) did not differ from controls in their scratching behavior. fMcpt6-/- and Cpa3Y356L,E378A mice also spent more time scratching after ET-1 injection than controls. g But no difference was seen in the mean length of scratching episodes. h The results from (e) analyzed over time. Both Mcpt6-/- and Cpa3Y356L,E378A mice scratched more than controls over the duration of the test (indicated by vertical significance bar). Further analysis showed that Cpa3Y356L,E378A mice scratched more than controls in the intervals between 10 and 40 min (indicated by stars), and Mcpt6-/- mice scratched significantly more between 10 and 30 min (indicated by #). Results are presented as mean ± SEM. #/*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001; n.s, not significant; Student’s t test (a-c); 2-way ANOVA with Bonferroni multiple comparison (d, h);

1-way ANOVA (e-g). Statistical outliers as identified by Grubb’s test are indicated with the symbol §

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