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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Glioblastoma hijacks microglial gene expression to support tumor growth

Fig. 1

Glioblastoma-interacting microglia internalized tumor-derived GFP. a A schematic illustrating our model of C57BL6.CCR2RFP/WT mice implanted with GL261.BpalmGFP glioblastoma cells. Four weeks after tumor implantation, brains were harvested, and microglia, monocytes, and macrophages were sorted based on cell specific antigens and GFP uptake. b IBA-1 positive microglia were present throughout the brain (1) and infiltrated the GFP-positive tumor (2–5). CCR2-positive (RFP-labeled) myeloid-derived cells infiltrated the tumor, but were mostly absent in other parts of the brain (1). c Confocal microscopy images show that GFP was taken up by IBA-1 positive microglia. d Microglia were identified as CD11bhigh/CD45med cells (dark blue gate). Microglia were then sorted based on the GFP signal detected as the upper limit in the control (no tumor) and GL261 wildtype (no GFP) implanted mice. Only in mice implanted with GL261.BpalmGFP, a population of GFP-positive microglia, was identified (green gate in the GFP/CCR2 plot). e Delineation of brain areas separated for microglial isolation in F. f Only microglia isolated from the tumor contained GFP. Results from a representative experiment are shown. g MAplot shows 384 significantly up- or downregulated genes plotted in red when comparing GFPpos (glioblastoma-interacting microglia-GIM) to GFPneg microglia. Scale bars b 1000 μm, 1–2; 200 μm, 3–5; 100 μm (c), 5 μm

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