Fig. 6

Quantification of cornea and spleen flow cytometry data in WT and rTg4510 cohorts. a There was a significantly lower frequency of CD45⁺ leukocytes in rTg4510 corneas compared to WT mice. b Representative dot plots demonstrate the shift in the “activated” DC phenotypes (CD80⁺ and CD86⁺) that are more prominent in rTg4510 cohorts. c Unstained samples of cornea and spleen tissues show the negative populations thus serving as a guide for identifying the positive population of CD80/CD86. d, e In the CD11c⁺CD11b⁺ corneal DC subset, the proportion of CD80⁺ and CD86⁺ DCs was reduced in WT mice compared to rTg4510 mice (P < 0.05). f, g Similar declines in the CD80⁺ and CD86⁺ DC populations were observed in CD11c⁺CD11b⁻ subset (P < 0.05). h–l Comparing WT and rTg4510 spleen tissues, no significant change in CD45, CD80, and CD86 populations was found in splenic CD11c⁺ or CD11b⁺ immune cell subsets (P > 0.05). Data are shown as mean ± SEM, where * indicates P ≤ 0.05 (n = 3 per group for corneas and n = 4 per group for spleens) as determined using the unpaired Student's t-test