Fig. 1

Acute inflammation differentially regulates the expression of LPARs and ENPP2 and increases LPA concentrations in mouse brain. a Schematic diagram of the experimental procedure. Mice (n = 7 per group) were injected intraperitoneally (i.p.) with PBS or LPS (5 mg/kg body weight). After 24 h, the brain and blood was collected and processed for further analyses. b One brain hemisphere from each mouse was processed for RNA isolation and further analysis by qPCR. Expression ratios were normalized to HPRT, and results were analyzed using the relative expression software tool (REST; **p < 0.01, ***p < 0.001; pairwise re-allocation test). The dotted lines indicate 2-fold up- or down-regulation. An LPA ELISA was utilized to quantitate LPA in the brain (n = 7 mice per group; (c)) and serum (n = 5 mice per group; (d)). Results are expressed as mean ± SD (*p < 0.05, **p < 0.01; unpaired Student’s t test)