Fig. 2
Effect of RRMS sera and matching sex and age HD on PBMCs migration on a normal endothelium. Freshly isolated PBMCs from healthy or RRMS subjects (n = 6/group) were put in contact for 4 h with hCMEC/D3 monolayer on Transwell polycarbonate filters in the presence of culture medium supplemented with 20% serum (16 h) from autologous healthy subject (control) or naïve-to-treatment RRMS patient (serum switch effect) (n = 6 in duplicate). PBMCs from RRMS patients were placed in contact with autologous RRMS patient serum (control) or healthy subject serum (serum switch effect). a Schematic diagram of experiment. After 4 h, the number of (b) CD4+RoRγt+ (TH17) and (D) CD4+Foxp3+ (Treg) migrated was evaluated by FACS analysis. Data are expressed as fold increase (% of relative control). Statistical analysis are performed by using Mann-Whitney U test (two tails) (mean ± s.e.m.); *P ≤ 0.05