Fig. 4
From: Inhibition of formyl peptide receptors improves the outcome in a mouse model of Alzheimer disease
Boc2-treatment ameliorates microglial cell reactivity in APP/PS1 double-transgenic mice. Coronal brain sections of 29-week-old APP/PS1 double-transgenic or WT mice with or without Boc2 treatment were stained with anti-IBA-1 as a marker for microglial cells. Quantification of IBA-1-staining intensities within (a) the hippocampus or (b) the cortex (cell density/mm2). c Representative images of the dentate gyrus. d Microglia reactivity determined by detailed morphological analysis (i.e., ramification index, RI). Analysis of the RI (Ap/Ac) as a subtle indicator for microglia activation revealed a decrease of RI in APP/PS1 double-transgenic compared to WT mice, which was less severe in Boc2-treated APP/PS1 double-transgenic mice. e Analysis of the RI (Ap/Ac) in the hippocampus. Scale bars: (c) 50 μm; (e) 5 μm. Ap projection area Ac cell area. Statistical significance was determined using two-way ANOVA with turkey test (A/B) or Kruskal-Wallis followed Dunn test (d). Data represent mean + SEM; n ≥ 5; *p < 0.05, **p < 0.01, ***p < 0.001