Fig. 2

Genetic deletion of CD11b suppresses P + M-induced microglial activation in mice. a Microglial cells in the brainstem of P + M-treated WT and CD11b KO mice were immunostained with antibody against Iba-1, and the representative images were shown. Activated microglia are characterized by enlarged cell bodies and high staining density. b The density of Iba-1 immunostaining in hippocampus was quantified. c, d The gene expressions of TNFα (c) and IL-1β (d) were determined in the brainstem of mice by using RT-PCR. n = 6 for each group. *p < 0.05. **p < 0.01. Scale bar = 50 μm