Fig. 3

Genetic deletion of CD11b attenuates P + M-induced activation of NLRP3 inflammasome in mice. a The expressions of NLRP3, pro-caspase-1, caspase-1 p10, pro-IL-1β, and IL-1β in the brainstem of P + M-treated WT and CD11b KO mice were detected by using Western blot, and the representative blots were shown. GAPDH was used as an internal control. b–f The band density of NLRP3 (b), pro-caspase-1 (c), caspase-1 (d), pro-IL-1β (e), and mature IL-1β (f) was quantified. n = 6 for each group. g The levels of IL-1β in the brainstem of mice were measured by using a commercial ELISA kit. *p < 0.05. **p < 0.01