Fig. 1

Experimental outline. Schematic representation of the study showing the 7 experimental groups coinciding with different time points before, during, and after cuprizone diet. MRI data was performed longitudinally at every time point in the same mouse cohort (continuous blue line). New cohorts of mice were used at each time points to assess locomotor-, anxiety-like behavior, and memory abilities. All mice underwent the same tests and some of them were used for histological evaluation at every time point. Additional cohorts were used to assess effects of Pavlovian conditioning paradigm only at the indicated time points