Fig. 3
From: Myelination- and immune-mediated MR-based brain network correlates
Structural and anatomical gray matter changes during de- and remyelination in the cuprizone model. Example immunohistochemical images of coronal mouse slices containing the neocortex (Cx) in control conditions (1st column), at 6 weeks after starting the cuprizone diet (cupri 6 weeks—full demyelination, 2nd column), and at full remyelination 6 weeks after reintroduction of normal food (3rd column). On the right of each panel, bar graphs show quantifications of changes. Stained for: a myelin specific marker PLP; b astrocytic specific marker GFAP; c exemplary T2-weighted images obtained in living mice during a longitudinal MRI scan. Pictures show frontal part of mouse brain containing neocortex, hippocampus, and CC. Bar graph shows the ratio calculated between the intensity of the myelin signal observed in caudal regions of the Cx (SICx) and CC (SICC). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001