Fig. 3

Increased levels of histamine receptor 2 (HR2) measured in the intestinal samples of young and aged mice for 6-h, 24-h, and 7-days post-stroke compared to the age matched controls. a mRNA expression levels of HR2 quantified by qPCR in young and aged mice at 6 and 24 h post-stroke. b (i) Protein levels of HR2 quantified by IHC followed by image J quantification in aged mice at 7 days post-stroke and c mRNA levels of HR2 quantified by RNA in situ hybridization and analyzed by image-J in the intestine of Ag mice at 7 days post-stroke. n = 3 (sham) and 5 (stroke) per group (a) and 5 (sham) and 5 (stroke) per group (b and c). Data are expressed as mean ± SEM (a), (b), and (c), as well as individual values. *P < 0.05, **P < 0.01, ***P < 0.001. P values were calculated using one-way analysis of variance with Tukey multiple comparisons correction (a) or with two-tailed unpaired Student’s t test (b and c). b Brown-HR2 protein; blue-Nuclei (b) purple dots (red arrows)-mRNA HR2