Fig. 5

Inhibition of FXR by Z-GS attenuated neuronal apoptosis through reducing OGD-induced calcium influx. a Cell viability of primary neurons that subjected to OGD and treated with different concentration of Z-GS and GW4064. n = 4–5 per group. *p < 0.05. b Double staining of TUNEL (red) and DAPI (blue) of neurons that subjected to OGD and treated with Z-GS (OGD + Z-GS) or GW4064 (OGD + GW4064), Bar = 50 μm. Data are presented as mean ± SD. n = 5 per group. *p < 0.05, ***p < 0.001. c Calcium images of neurons that subjected to OGD after treatment with Z-GS or IM (50 nM), Bar = 50 μm, n = 4–5 per group. *p < 0.05, **p < 0.01, ***p < 0.001. d Staining of TUNEL (red) of neurons that subjected to OGD after treatment with Z-GS and ionomycin (IM), Bar = 50 μm. Data are presented as mean ± SD. n = 4–5 per group. *p < 0.05, ***p < 0.001