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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Serum IgG-induced microglial activation enhances neuronal cytolysis via the NO/sGC/PKG pathway in children with opsoclonus-myoclonus syndrome and neuroblastoma

Fig. 8

The exaggeration of neuronal cytolysis by pretreatment with pharmacological activators of the NO pathway. Note that the cytolysis of cerebral cortical neurons (a) and cerebellar neurons (b) induced by conditioned media from microglias treated with OMS + NB IgG was exacerbated by the NO-donor SNAP, and the cytolysis of cerebral cortical neurons (c) and cerebellar neurons (d) was also upregulated by YC-1, an activator of sGC. Similarly, the PKG activator 8Br-cGMP had exaggerated effects on the cytolysis of cerebral cortical neurons (e) and cerebellar neurons (f). The exaggerated effects of SNAP, YC-1, or 8Br-cGMP and neuronal cytolysis induced by conditioned media were suppressed by pretreatment with minocycline, an inhibitor of microglias (af). ***p < 0.001, one-way ANOVA, n = 20 (vehicle, NB; activator, NB), n = 10 (vehicle, OMS + NB; activator, OMS + NB; saline, activator, OMS + NB; minocycline, activator, OMS + NB)

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