Fig. 1
From: Stress-induced NLRP3 inflammasome activation negatively regulates fear memory in mice
NLRP3 inflammasome is activated in electric foot shock induced contextual fear memory. a, b Representative image of immunoblotting and quantitative analysis of cleaved caspase-1, NLRP3, and β-actin in the hippocampus collected from control mice (no electric foot shock) and fear conditioned mice at 3 h post the electric foot shocks. Control (Ctrl), n = 7; electric foot shocks (FS), n = 9. c-e qPCR analysis of IL-1β, NLRP3, and TNF-α mRNA levels in isolated microglia collected from control and fear conditioned mice at 3 h post the electric foot shocks. c, dn = 4 per group. e Ctrl, n = 3; FS, n = 5. f Heatmap of differential expression genes related to neuronal functions in hippocampus collected from control mice and fear conditioned mice at 3 h and 72 h post electric foot shocks. g, h GSEA of toll-like receptor signaling and RIG-I-like receptor signaling, FS vs Ctrl. i Heatmap of differential expression genes related to inflammation at 72 h post the electric foot shocks. Data shown are mean ± SEM. Data were analyzed by t test (b-e). NS, no significance, *p < 0.05, **p < 0.01, ***p < 0.001