Fig. 5

Activating CD200/CD200R pathway upregulated synapse-related proteins expression after ischemic stroke in rats. Representative western blots of PSD-95 and synaptophysin (SYP) and their corresponding normalized control β-actin ipsilateral sensorimotor cortex at 4 days, 7 days, and 28 days after MCAO (a). CD200Fc increased the protein levels of PSD-95 at 4, 7, and 28 days and the protein levels of SYP at 28 days after MCAO in ipsilateral sensorimotor cortex (b, c) (n = 3). CD200R Ab reduced the protein levels of SYP at 4 days after MCAO in ipsilateral sensorimotor cortex (c) (n = 3). Dendritic spines’ density and morphology were detected by Golgi-Cox staining at 28 days after MCAO (d–f) (n = 4, scale bar: 5 μm). Results are expressed as means ± SEM. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 versus the Sham group. ^**P < 0.05, ^**P < 0.01, ^***P < 0.001 versus the MCAO+IgG group