Fig. 3
From: Function of astrocyte MyD88 in high-fat-diet-induced hypothalamic inflammation
Palmitic acid–induced hypothalamic gliosis is attenuated by ablation of Myd88 expression in astrocytes. To identify the effect of astrocyte MyD88 on saturated free fatty acid–induced hypothalamic reactive gliosis, astrocyte-specific Myd88 KO mice (Myd88△GFAP) and control Myd88fl/fl mice were icv administered palmitic acid (50 pmol/2 μl), and their astrocytes and microglia were immunohistochemically analyzed with GFAP and Iba1 antibodies. a, d Representative images show palmitic acid–induced changes in hypothalamic GFAP-positive cells (a) and Iba1-positive cells (d) in Myd88fl/fl mice and Myd88△GFAP mice. b, c, e, f Number and intensity of GFAP-positive cells (b, c) and Iba1-positive cells (e, f) observed in the hypothalamic ARC of Myd88fl/fl mice and Myd88△GFAP mice after icv injection of palmitic acid or vehicle (n = 4–6 sections of 2–3 mice/group). Data are presented as mean ± SEM. *p < 0.05 and ***p < 0.001. ns, not significant. Scale bar = 100 μm