Fig. 2

Deletion of TDAG8 gene attenuates mechanical and thermal hyperalgesia and reduces arthritis scores. TDAG8+/+, +/−, −/− mice (8–12 weeks old) were injected with 5 μl of 100% CFA (5 μg) in the right ankle joint (ipsilateral joint) four times at 1-week intervals, then underwent mechanical (a, b) or thermal (c, d) behavioral tests. Arrows are CFA treatments. Data are mean ± SEM of total tested mice. *p < 0.05, **p < 0.01, ***p < 0.001, TDAG8^−/− (n = 5) vs TDAG8^+/+ (n = 3), or TDAG8^−/− (n = 10) vs TDAG8^+/− (n = 3). e Severity of arthritis presented as arthritis score. *p < 0.05, **p < 0.01, ***p < 0.001, TDAG8^−/− (n = 10) vs TDAG8^+/+ (n = 3) by two-way ANOVA. f IL-6 production at 12 w after the first CFA injection in TDAG8^+/+ and TDAG8^−/− mice. ***p < 0.001, TDAG8^−/− (n = 3) vs TDAG8^+/+ (n = 3) by Mann-Whitney U test. g IL-17 production at 8 and 12 weeks after the first CFA injection in TDAG8^+/+ and TDAG8^−/− mice. *p < 0.05, TDAG8^−/− (n = 6) vs TDAG8^+/+ (n = 4) by two-way ANOVA