Fig. 3
Histology of ipsilateral side joints stained with hematoxylin and eosin or with macrophage markers in TDAG8+/+ or TDAG8−/− RA mice. A–D Histology of joints from TDAG8+/+ or TDAG8−/− RA mice at week 12 stained with hematoxylin and eosin. A Inflamed joint images (a, e). a for TDAG8+/+, e for TDAG8−/−; the small letters (b, c, d, f, g, h) in the inflamed joint images represent the regions that were amplified in pannus (b, f), bone erosion (c, g) and cartilage damage (d, h) images. (B–D) Data are mean ± SEM severity score for pannus (B), bone erosion (C), and cartilage damage (D). **p<0.01 TDAG8−/− vs TDAG8+/+ by Mann-Whitney U test. (E–H) Histology of joints from TDAG8+/+ or TDAG8−/− RA mice at week 12 stained with CD80 (F), CD163 (G), or CD68 (H) antibodies. Synovial sublining regions are shown in (E). Black arrows are labeled cells. The cell density (cells/mm2) was presented in histograms representing the mean ± SEM (F–H). *p < 0.05, TDAG8−/− vs TDAG8+/+ by Mann-Whitney U test