Fig. 9
CCL-2d or LCC-09 suppresses TDAG8 expression and function and reduces the number of SGCs in DRG. a DRG from control (Ctrl) or compound-treated mice were taken at 0 and 12 weeks after RA induction, then underwent qRT-PCR to measure TDAG8 mRNA expression. Data are mean ± SEM. ***p < 0.001 Ctrl 12 weeks vs Ctrl 0 weeks; ##p < 0.01, ###p < 0.001, compound-treated 12 weeks vs Ctrl 12 weeks by one-way ANOVA. b HEK293 cells were transfected with TDAG8, then incubated with 0.1, 1, and 10 μM CCL-2d or LCC-09, followed by acid (pH 5.5) stimulation. Calcium signals were detected. Data are mean±SEM peak values of signals. n = 20–47 cells. c–f DRG from vehicle- (c–f), CCL-2d- (d), or LCC-09 (e)-treated mice were taken at 12 weeks, then co-immunostained with anti-GFAP and anti-PERI antibodies. Cell images (c, d) showing GFAP- (green) and PERI (red)-positive neurons. Scale bar is 50 μm. (e, f) Histograms represent the mean ± SEM percentage of PERI-positive neurons surrounded by GFAP+ cells (PERIGFAP+) to total PERI-positive (PERIT) neurons. *p < 0.05 by the z test