Exp. | Strain | Induction of EAE | Group | Treatment | Incidence of EAE | Mean cumulative score | Mean maximal score | % Maximal weight loss |
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1 | C57BL/6 | Active | a | GMCSF-MOG | 2 of 8 | 2.1 ± 1.5 | 0.3 ± 0.2 | − 0.8 ± 2.1 |
1 | C57BL/6 | Active | b | GMCSF-OVA | 7 of 7 | 81.1 ± 6.9 | 3.9 ± 0.1 | 17.4 ± 4.1 |
2 | 2D2-FIG-Rag1−/− | Vaccine | c | GMCSF-MOG | 3 of 6 | 49.0 ± 22.4 | 1.7 ± 0.8 | 6.4 ± 4.3 |
2 | 2D2-FIG-Rag1−/− | Vaccine | d | GMCSF-NFM | 5 of 6 | 48.3 ± 16.9 | 2.6 ± 0.5 | 4.4 ± 1.6 |
2 | 2D2-FIG-Rag1−/− | Vaccine | e | Saline | 0 of 5 | 0.0 ± 0.0 | 0.0 ± 0.0 | − 2.7 ± 0.9 |
2 | 2D2-FIG | Vaccine | f | GMCSF-MOG | 0 of 6 | 0.0 ± 0.0 | 0.0 ± 0.0 | − 4.0 ± 2.8 |
2 | 2D2-FIG | Vaccine | g | GMCSF-NFM | 0 of 6 | 0.0 ± 0.0 | 0.0 ± 0.0 | − 2.4 ± 1.8 |
2 | 2D2-FIG | Vaccine | h | GMCSF-MOG + GMCSF-NFM | 0 of 6 | 0.0 ± 0.0 | 0.0 ± 0.0 | − 0.7 ± 2.2 |
2 | 2D2-FIG | Vaccine | i | Saline | 0 of 6 | 0.0 ± 0.0 | 0.0 ± 0.0 | − 1.6 ± 0.9 |
- Experiment 1: C57BL/6 mice were treated with 2 nmol GMCSF-MOG (n = 8) or GMCSF-OVA (n = 7) on days − 21, − 14, and − 7. On day 0, all mice were immunized with 200 μg MOG35–55 in CFA and 400 ng of Pertussis toxin i.p. on days 0 and 2. The % maximum weight loss between days 1 and 40 was analyzed with a two-tailed Student’s t test. (a) versus (b), (p ≤ 0.001). Negative values reflect weight gain. Mean cumulative and maximal scores were analyzed with a Mann-Whitney U test (a) versus (b), (p ≤ 0.001)
- Experiment 2: 2D2-FIG (n = 6) or 2D2-FIG-Rag1−/− (n = 5–6) mice were vaccinated with 4 nmol GMCSF-MOG, GMCSF-NFM, “GMCSF-MOG + GMCSF-NFM”, or with saline on days 0 and day 20. The mean cumulative and maximal scores were analyzed with a Kruskal-Wallis Test and adjusted by the Bonferroni correction. Mean cumulative scores (d) versus (e–i), (p < 0.01) and mean maximal scores (c) versus (f–i) (p < 0.05) and (d) versus (e–i), (p < 0.01)