Fig. 8

Spinal microglia is not involved in HSD-induced mechanical hypersensitivity in female mice. a Minocycline significantly reduced the number of Iba-1⁺ microglia in the spinal cord dorsal horns in both male and female mice, n = 3/group. Scale bar: 100 μm. The outline indicates the area of interest (lamina I–III) where microglia number was quantified. b Minocycline increased the paw withdrawal thresholds in male HSD mice, but not in female HSD mice. Data with ND-fed mice was included (green dotted line) as reference. N = 6–8/group. All data was presented as mean ± SEM and analyzed with an unpaired t test for a or a two-way ANOVA followed by Bonferroni post-tests for b. **p < 0.01, ***p < 0.001