Fig. 6

Rottlerin affects the PMA-induced MMP-9-mediated astrocytic migration. Cells were plated on 6-well culture plates, grew to confluence, and starved with serum-free medium for 24 h. Cells were pretreated with MMP-9 inhibitor (2/9i, 1 μM) or Rott (1 μM) for 1 h, and the monolayer cells were manually scratched with a blue tip as described in the “Methods” section and then incubated with PMA (1 μM) for 24 h. Phase contrast images of cells were taken at 24 h (upper panel), and the number of cell migration was counted (lower panel) as described in the “Methods” section. Data are expressed as mean ± SEM of three independent experiments (N = 3). ^#P < 0.01, as compared with the respective values of untreated control. **P < 0.01, as compared with the values of cells stimulated with PMA only