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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Evaluation of M2-like macrophage enrichment after diffuse traumatic brain injury through transient interleukin-4 expression from engineered mesenchymal stromal cells

Fig. 2

Functionality of IL-4 expressed by IL-4 mRNA-transfected MSCs. a IL-4 concentration in either MSC conditioned media (CM) that was supplemented with 100 ng/mL recombinant IL-4 (rIL-4 CM) or CM from MSCs transfected with IL-4 mRNA (eIL-4 CM); ns, not significant. b Flow plots demonstrating CD206 expression after gating for single macrophages. c Percentage of J774A.1 macrophages expression CD206 after treatment with differently conditioned media. Analyzed in a flow cytometer with a fluorescent CD206 antibody. Both rIL-4 CM and eIL-4 CM were significantly different from Media and naïve MSC CM. d Percentage of macrophages expression CD163 after treatment with differently conditioned media. Mean ± SD, Student’s t test, and one-way ANOVA with Tukey’s post hoc test; ****p < 0.0001

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Journal of Neuroinflammation

ISSN: 1742-2094