Fig. 4

Exaggerated IL-1β and 25-HC production in LPS-activated microglia expressing human ApoE4. The levels of IL-1β or IL-6 secreted into the medium in apoE2- or apoE4-expressing microglia or apoE KO microglia treated with LPS (10 ng/ml) and 25-HC (0, 1, 2, or 10 μg/ml) for 6 h (a) or 24 h (b and c). The levels of IL-1β secreted in the medium of apoE3- or apoE4-expressing microglia after 24 h treatment with LPS (10 ng/ml) and 25-HC (10 μg/ml) (d, e) and the levels of 25-HC in these cells or medium were determined by GC-MS (f). Statistical significances were determined by two-way ANOVA with multiple comparisons in a, b, c, d, or unpaired Student t test in f. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001, respectively. The data shown are representative of three or more independent experiments