Fig. 5

MiR-669c overexpression does not change overall microglia/macrophage activation under cerebral ischemia. Total microglial/macrophage activation was assessed by quantification of Iba1 immunostaining in the ipsilateral hemisphere. Iba1 immunoreactivity was not altered by the miR-669c-3p-mediated (669) overexpression at 3 dpi (a). Panels b and c are representative photographs of coronal sections stained with Iba1 in LV1-GFP control (b) and LV1-miR-669c injected tMCAo animals (c). One-way ANOVA followed by Bonferroni’s post hoc tests: *p < 0.05 compared to the respective sham-operated animals. N = 3 in each sham and N = 6 in each tMCAo group. Panels d and e depict the extent of GFP expression in the brains of the LV1-GFP (d) and LV1-miR-669c (e) injected stroke animals. Panels f-m contain confocal microphotographs depicting LV transduced, GFP⁺ cells colocalizing with Iba1⁺ cells in the ipsilateral striatum of LV1-GFP control (f-i) and LV1-miR-669c (j-m) stroke mice