Fig. 9

MiR-669c-3p is directly targeting MyD88 transcript in vitro and decreasing MyD88 immunoreactivity in ischemic stroke in vivo. The expression analysis of MyD88 has shown its downregulation in LPS-challenged miR-669c overexpressing BV2 cells (a). MiRNA pulldown results revealed that miR-669c-3p directly targets MyD88 (b), MMP9 (c), and TNF-α (d) transcripts in LPS exposed BV2 cells. MyD88 transcript also was shown to be targeted by miR-669c-3p in N2a cells exposed to OGD/R (e). The expression analysis of MyD88 confirmed its downregulation in miR-669c overexpressing N2a cells subjected to OGD/R (f). Unpaired two-tailed t tests: **p < 0.01, ***p < 0.001 compared to LV1-GFP transduced BV2 or N2a cells, ***p < 0.001 compared to control miR-39-3p transfected BV2 or N2a cells. N = 3-4 in each group. The extent of MyD88 ipsistriatal immunoreactivity was decreased in LV1-miR-669c injected stroke mice (g). Panels h-m depict typical examples of MyD88 immunoreactivity in LV1-GFP (h, j, l) and LV1-miR-669c (i, k, m) injected stroke animals. Unpaired two-tailed t test: *p < 0.05 compared to LV1-GFP stroke mice. N = 6 in each stroke group