Fig. 12

The α-diversity of the fecal microbiome among seven groups according to Ace (a), Chao 1 (b), and Shannon index (c). Data are presented as mean ± SD. One-way analysis of variance (ANOVA) was performed and post hoc comparisons were carried out using the LSD test. Asterisks indicate significant differences. *P < 0.05; **P < 0.01. The β-diversity of the fecal microbiome among five groups according to unweighted UniFrac distance (d). Each box plot represents the median, interquartile range, minimum, and maximum values. Groups: WT: wild type control group of C57BL/6J mice; APP/PS1 Con: transgenic control group of APP/PS1 mice; WT 27-OHC: C57BL/6J mice treated with 5.5 mg/kg 27-hydroxycholesterol; APP/PS1 27-OHC: APP/PS1 mice treated with 5.5 mg/kg 27-hydroxycholesterol; APP/PS1 ANS: APP/PS1 mice treated with 0.2 mg/day anastrozole; WT CO: C57BL/6J mice treated with 5.5 mg/kg 27-hydroxycholesterol plus 0.2 mg/day anastrozole; APP/PS1 CO: APP/PS1 mice treated with 5.5 mg/kg 27-hydroxycholesterol plus 0.2 mg/day anastrozole. n = 6/group