Fig. 17

Relative mRNA levels (a) and Western blot analyses (b) of OLCD, relative mRNA levels (c) and Western blot analyses (d) of CLOD-1, relative mRNA levels (e) and Western blot analyses (f) of CLOD-5, and relative mRNA levels (g) and Western blot analyses (h) of ZO-1 expression for different groups. Protein bands for Western blot analyses of tight junction protein expression for different groups in iluem (I) and colon (j). Groups: WT: wild-type control group of C57BL/6J mice; APP/PS1 Con: transgenic control group of APP/PS1 mice; WT 27-OHC: C57BL/6J mice treated with 5.5 mg/kg 27-hydroxycholesterol; APP/PS1 27-OHC: APP/PS1 mice treated with 5.5 mg/kg 27-hydroxycholesterol; APP/PS1 ANS: APP/PS1 mice treated with 0.2 mg/day anastrozole; WT CO: C57BL/6J mice treated with 5.5 mg/kg 27-hydroxycholesterol plus 0.2 mg/day anastrozole; APP/PS1 CO: APP/PS1 mice treated with 5.5 mg/kg 27-hydroxycholesterol plus 0.2 mg/day anastrozole. One-way analysis of variance (ANOVA) was performed and post hoc comparisons were carried out using the LSD test. Asterisks indicate significant differences. Data are presented as mean ± SEM. *P < 0.05; **P < 0.01. n = 6/group