Fig. 6

Immunoblot analysis of proteins extracted from cerebral cortex from CHOW and MCD mouse groups. a Proteins resolved MAPK activation markers phospho and total ERK1/2 and p38α/β and NF-κβ activation marker phospho-p65 and total p65 in b. c Densitometric analysis of phospho ERK½ and total ERK½ is displayed as mean ± SD (n = 3). d Densitometric analysis of p38α/β and NF-κβ activation marker phospho-p65 and total p65 normalized against β-actin and plotted as bar graph. e Co-localization and morphometric analysis p47Phox/gp91Phox as shown by immunofluorescence imaging in brain sections from CHOW and MCD fed mouse groups. Co-localization was displayed yellow dots, indicated by white circles. Images were taken at × 20 magnification. f immunoreactivity was displayed as bar graph representing mean ± SD of ROI % of 3 different areas (n = 3). Significance was tested by unpaired t test,*p < 0.05, **p < 0.01, ***p < 0.001. g mRNA expression analysis of TLR4, RAGE, and p47 Phox in cerebral cortex of CHOW and MCD fed mouse groups. mRNA expression was assessed by quantitative real-time PCR, and expression was normalized against CHOW diet group, *p < 0.05, **p < 0.01, ***p < 0.001