Fig. 2

NF-κB/cytokine mRNA expression profiles of ASD patient and healthy control PBL treated with DNAoxy. PBL from control subjects and ASD patients were incubated with DNAoxy (50 ng/mL) for 3 and 24 h. Transcript levels for NF-κB, IL-1β, IL-8, and TNFα were evaluated by RT-qPCR analysis. Samples were normalized according to ТВР and GAPDH expression. The mRNA abundance is expressed relative to NF-κB/cytokine mRNA level in intact PBL of healthy controls (horizontal green line, 1.0 ± 0.3 arb.un). Data are shown as means ± SD. a Control, PBL from healthy children. b Group 1, PBL from patients with mild-to-moderate ASD; *p value < 0.01 compared to intact PBMC of healthy controls (Mann–Whitney U test)