Fig. 6

Double immunofluorescence analysis of the relationship between T lymphocytes and astroglial and microglial cells in the brains of ⍺-synuclein transgenic mice. a Split and merged representative laser scanning confocal microscopy images from the neocortex, hippocampus, striatum, and thalamus of non-tg and ⍺-syn tg mice double labeled with antibodies against CD3 cells (T cells, red channel) and GFAP (astroglial cells, FITC channel). As shown, in the ⍺-syn tg mice astroglial processes are in closer proximity to CD3 positive cells. b Computer-based image analysis showing significant increase in the average number of astroglial processes in close proximity to CD3 positive cells in neocortex, hippocampus, and striatum of ⍺-syn tg mice. c Split and merged representative laser scanning confocal microscopy images from the neocortex, hippocampus, striatum, and thalamus of non-tg and ⍺-syn tg mice double labeled with antibodies against CD3 cells (T cells, red channel) and Iba1 (microglia, FITC channel). As shown, in the ⍺-syn tg mice astroglial processes are in closer proximity to CD3 positive cells. d Computer-based image analysis showing significant increase in the average number of microglial processes in close proximity to CD3 positive cells in neocortex, hippocampus, and striatum of ⍺-syn tg mice. Scale bars = 10 μm (low magnification) and 5 μm (high magnification). Non-tg and α-syn tg mice (n = 4). Statistical significance determined by unpaired t test; *p ≤ 0.05