Fig. 7

Double immunofluorescence analysis of the relationship between T lymphocytes and neuronal cells displaying accumulation of ⍺-synuclein in transgenic mice. a Split and merged representative laser scanning confocal microscopy images from the neocortex, hippocampus, striatum, and thalamus of non-tg and ⍺-syn tg mice double labeled with antibodies against CD3 cells (T cells, red channel) and human ⍺-syn (Lewy body-like aggregates, FITC channel). As shown, in the ⍺-syn tg mice, there are occasional CD3-positive cells in close proximity to neurons displaying accumulation of ⍺-syn. b Computer-based image analysis showing decrease in the average distance between CD3 cells to neurons displaying accumulation of ⍺-syn in the striatum of ⍺-syn tg mice. Scale bars = 10 μm (low magnification) and 5 μm (high magnification). Non-tg and α-syn tg mice (n = 4). Statistical significance determined by unpaired t test; *p ≤ 0.05