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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Three-dimensional morphometric analysis reveals time-dependent structural changes in microglia and astrocytes in the central amygdala and hypothalamic paraventricular nucleus of heart failure rats

Fig. 2

HF-induced morphometric changes in CeA microglia. a Representative confocal images show IBA1-stained microglia in the PVN of HF and sham rats 16 weeks post-surgery. b Brain scheme shows the topographic location of CeA microglia that have been used for the morphometric assessment. The red dotted line highlights the CeA. c Dot-plot graphs show the individual values of CeA microglia for surface area, cell volume, filament length, microglial branches, microglial segments, filament terminals, and IBA 1 intensity for sham rats (N = 1070 cells from 12 rats, pooled) and HF rats at 8, 14, and 16 weeks post-surgery (N = 363 cells from 4 rats, N = 355 cells from 4 rats and N = 332 cells from 4 rats, respectively). *p < 0.05, **p < 0.01, and ***p < 0.0001 vs. respective sham, one-way ANOVA followed by Tukey’s post hoc test. d Plot graphs depicting cell volume, filament length, and number of microglial branches as a function of %EF values combining sham (n = 4), HF rats (EF < 50%, n = 4) and mild HF rats (EF > 50%, n = 5). R2 and p values were obtained following a Pearson correlation analysis

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