Fig 5

HF-induced morphometric changes in PVN and CeA astrocytes. a Representative confocal image showing PVN astrocytes in a sham rat stained with GFAP (red) and GluSyn (green). b Dot-plot graphs show the individual values of PVN microglia for surface area, cell volume, filament length, and IBA1 intensity in sham (n = 4) and HF rats 14 weeks post-surgery (n = 4). c Bar graph shows the mean number of total sholl intersections in PVN astrocytes in sham (n = 4) and HF rats 14 weeks post-surgery (n = 4). d Representative confocal image showing CeA astrocytes stained with GFAP (red) and GluSyn (green) in a sham rat. e Dot-plot graph show the individual values of CeA microglia for surface area, cell volume, filament length, and IBA1 intensity in sham (n = 4) and HF rats 14 weeks post-surgery (n = 4). f Bar graph shows the mean number of total Sholl intersections in CeA astrocytes in sham (n = 4) and HF rats 14 weeks post-surgery (n = 4). g, h Mean distribution plots of the number of Sholl intersections as a function of the distance from the microglial soma for sham and HF rats 14 weeks post-surgery (n = 4/group). i Confocal images show a single PVN astrocyte from a sham animal before and after surface reconstruction with IMARIS. *p < 0.05, **p < 0.01, and ***p < 0.0001 vs. respective sham, Student’s t test, or two-way ANOVA