Three-dimensional morphometric analysis reveals time-dependent structural changes in microglia and astrocytes in the central amygdala and hypothalamic paraventricular nucleus of heart failure rats

Althammer, Ferdinand; Ferreira-Neto, Hildebrando Candido; Rubaharan, Myurajan; Roy, Ranjan K.; Patel, Atit A.; Murphy, Anne; Cox, Daniel N.; Stern, Javier E.

doi:10.1186/s12974-020-01892-4

Journal of Neuroinflammation

Table 1 Summary of microglial morphometric parameters of sham and HF rats for the PVN and CeA

From: Three-dimensional morphometric analysis reveals time-dependent structural changes in microglia and astrocytes in the central amygdala and hypothalamic paraventricular nucleus of heart failure rats

Brain region	Group	Cell volume (μm³)	Surface area (μm²)	Filament length (μm)	Microglial branches	Microglial segments	Filament terminals	IBA1 intensity (AU)
PVN	Sham 8 weeks N = 381 cells n = 4 animals	3157 ± 112	4048 ± 99	745 ± 21	104 ± 3	219 ± 7	116 ± 4	9449 ± 123
	Sham 14 weeks N = 365 cells n = 4 animals	3098 ± 119	4100 ± 107	736 ± 18	102± 4	224 ± 8	118 ± 6	9385 ± 135
	Sham 16 weeks N = 389 cells n = 4 animals	3181 ± 122	4068 ± 81	761 ± 25	100 ± 5	216 ± 7	117 ± 6	9522 ± 139
	HF 8 weeks N = 378 cells n = 4 animals	2838 ± 58	3868 ± 75	642 ± 12	82 ± 2	171 ± 5	90 ± 3	11138 ± 118
	HF 14 weeks N = 407 cells n = 4 animals	2212 ± 74	3424 ± 64	629 ± 35	78 ± 2	163 ± 7	85 ± 3	12764 ± 202
	HF 16 weeks N = 399 cells n = 4 animals	2012 ± 69	3233 ± 59	524 ± 39	72 ± 3	150 ± 5	83 ± 4	12783 ± 231
	HF 16 weeks EF > 50% N = 435 cells n = 5 animals	3234 ± 128	3988 ± 90	741 ± 27	97 ± 6	216 ± 8	115 ± 9	9433 ± 146
CeA	Sham 8 weeks N = 377 cells n = 4 animals	3588 ± 137	4885 ± 119	841 ± 28	109 ± 6	219 ± 7	114 ± 3	10134 ± 137
	Sham 14 weeks N = 339 cells n = 4 animals	3644 ± 122	4966 ± 131	865 ± 31	102 ± 2	214 ± 5	116 ± 4	10073 ± 144
	Sham 16 weeks N = 354 cells n = 4 animals	3691 ± 123	4904 ± 127	833 ± 24	104 ± 5	216 ± 7	114 ± 3	10285 ± 180
	HF 8 weeks N = 363 cells n = 4 animals	3634 ± 119	4700 ± 124	765 ± 29	106 ± 4	222 ± 7	112 ± 3	10344 ± 176
	HF 14 weeks N = 355 cells n = 4 animals	4418 ± 187	4964 ± 144	781 ± 28	92 ± 4	192 ± 8	100 ± 3	11314 ± 277
	HF 16 weeks N = 332 cells n = 4 animals	3180 ± 115	4395 ± 163	623 ± 24	89 ± 3	179 ± 6	94 ± 4	12779 ± 357
	HF 16 weeks EF > 50% N = 384 cells n = 5 animals	3540 ± 132	4798 ± 141	835 ± 56	94 ± 4	202 ± 7	110 ± 4	11322 ± 290
S1BF	Sham 14 weeks N = 139 cells n = 4 animals	4577 ± 138	6008 ± 234	967 ± 31	125 ± 5	261 ± 8	135 ± 7	10539 ± 183
S1BF	HF 14 weeks N = 162 cells n = 4 animals	4257 ± 130	5664 ± 224	957 ± 31	126 ± 4	264 ± 8	138 ± 8	10647 ± 171

Table shows individual values (mean ± SEM) of all analyzed microglial parameters, N = number of analyzed microglia, n = number of animals per group, CeA = central amygdala, PVN = paraventricular nucleus, S1BF = somatosensory cortex barrel field 1

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ISSN: 1742-2094

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