Fig. 3

IFNAR1 deficiency prevents damage to neuronal dendrites and microgliosis but not astrocytosis in HIVgp120tg mice. Representative images of the frontal cerebral cortex (a) immunolabeled for neuronal MAP2 (MAP2; layer 3; deconvolution microscopy; scale bar, 40 μm); astrocytic GFAP (cortex, all layers; fluorescence microscopy; scale bar, 40 μm), and Iba⁺ microglia numbers (cortex, all layers; fluorescence microscopy; scale bar, 100 μm). b–d Quantification of microscopy data obtained in the cortex and hippocampus of sagittal brain sections of 12–14-month-old mice. Neuropil positive for neuronal MAP2 (b); Fluorescence signal for astrocytic GFAP (c); Counts of Iba1⁺ microglia (d). Genotypes: WT (WT), HIVgp120tg (GP), IFNAR1KO (KO), and IFNAR1KO × gp120 (KOGP). Values are presented in combined box-dot plots with the 25th and 75th percentiles. The middle line of the box shows the median, and the mean is indicated by a “+”; ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05; ANOVA and Tukey’s HSD post hoc test; n = 6 animals (3 males and 3 females) per group/genotype (total n = 24 animals)