Fig. 8

IFNAR1 deficiency abrogates activity loss of ERK1/2 and upregulation of STAT1 in the hippocampus of HIVgp120tg mice. The expression level of phospho-ERK1 and phospho-STAT1 was assessed by Western blot analysis. Representative Western blot of phospho-ERK1/2, ERK1/2, and β-tubulin in the hippocampus (a). The graph shows the quantification of phospho-ERK and ERK by densitometry using normalization to GAPDH (b). Representative Western blot of phospho-STAT1, STAT1, and β-tubulin in the hippocampus (c). The graph shows the densitometry analysis of phospho-STAT1 and STAT1 normalized to GAPDH (d). Genotypes: WT (WT), HIVgp120tg (GP), IFNAR1KO (KO), and IFNAR1KO × gp120 (KOGP). Values are presented in combined box-dot plots with the 25th and 75th percentiles. The middle line of the box shows the median, and the mean is indicated by a “+”; number on the bar indicates P value, P < 0.05 as cut-off for significance; n.s., not significant; ANOVA and Tukey’s HSD post hoc test; brain tissues were derived from n = 6 of 11–14-month-old animals (3 males and 3 females) per group/genotype (total n = 24 animals)