Fig. 7

Decreased TREM2 protein levels in Tg-FDD mice microglia. a WB analysis and quantification of TREM2 protein levels in the cerebral hemisphere of WT and Tg-FDD mice. b Double immunofluorescence images of microglia (IBA1, red) and TREM2 (green) in the hippocampus of WT and Tg-FDD mice. c WB analysis and quantification of TREM2 protein levels in the cerebellum of WT and Tg-FDD mice. d Double immunofluorescence images of microglia (IBA1, red) and TREM2 (green) in the cerebellum of WT and Tg-FDD mice. For WB analysis (a and c), TREM2 levels were normalized by vinculin. For WB quantifications, error bars represented ± SEM n = 4, where *p < 0.05 of ***p < 0.001, unpaired Student’s t test. For double immunofluorescence (b and d), a decrease of TREM2 (green) positive microglia (IBA1, red) was observed in WT versus Tg-FDD mice in both brain regions (Merge). Colocalization analysis (CC) was performed to determine pixel intensity correlation between TREM2 and IBA1. White pixels indicate colocalization between TREM2 and IBA1 signal. All are representative images of 9-month-old WT or Tg-FDD mice. Scale bar 100 μm