Fig. 2

Tat reduces the expression of mortalin without altering its localization. a) percentage change in mRNA expression of mortalin in HIV-1 Tat-transfected astrocytes as analyzed by RT-PCR. GAPDH was used as the internal control (n = 5). b) Representative western blot showing decrease in mortalin level in HIV-1 Tat transfected astrocytes (n=5). c) Percentage change in mortalin level in HIV-1 Tat-transfected astrocytes as analzed by western blot. β-tubulin was used as the loading control. d) Bar graphs represent the percentage change in mortalin level in cytoplasmic extract of HIV-1 Tat transfected astrocytes. (Right panel) Representative western blot showing decrease in mortalin level in HIV-1 Tat transfected astrocytes in the cytoplasmic extract. e) Bar graphs represent the percentage change in mortalin level in nuclear extract of HIV-1 Tat transfected astrocytes. (Right panel) Representative western blot showing no change in mortalin level in HIV-1 Tat transfected astrocytes (n =3 ). Total histone3 (H3) was used as nuclear control, and GAPDH was used as cytoplasmic control. All data represent mean ± standard deviation, from independent experiments, **p< 0.005 (n.s. stands for non-significant. n indicates the number of independent experiments)