Fig. 4

Memantine reduces NR2B expression and inhibits apoptosis in IgG(AQP4+) mice. a Immunofluorescence staining of NR2B in the spinal cords of IgG(AQP4+) mice treated with memantine or vehicle at 1 dpi. IgG(con) mice treated with vehicle were used as a sham control. Sections were counterstained with DAPI. Scale bar = 100 μm. b Relative intensity of NR2B immunofluorescence in the spinal cord. n = 5 per group. Data are mean ± SEM. One-way ANOVA with Tukey-Kramer post hoc test. ns, not significant; ***p < 0.001. c TUNEL staining of apoptotic cells in the spinal cords of IgG(AQP4+) mice treated with memantine or vehicle at 1 dpi. IgG(con) mice treated with vehicle were used as a sham control. Sections were counterstained with DAPI. Scale bar = 100 μm. d Quantitative analysis of the number of TUNEL-positive cells in the spinal cord. n = 5 per group. Data are mean ± SEM. One-way ANOVA with Tukey-Kramer post hoc test. ns, not significant; ***p < 0.001