Fig. 1

AL002a promoted TREM2-dependent DAP12 and Syk phosphorylation. Panel a shows that AL002a binds to BMM derived from WT, but not Trem2 KO mice, as measured by FACS. A rat anti-Trem2 antibody from R&D Systems was used as a positive binding control and an isotype msIgG1 antibody as a negative control. Panel b: Following AL002a stimulation, WT or Trem2-/- BMM were lysed and immunoprecipitated with Trem2 antibody. Protein was loaded on a SDS gel in unreduced conditions. The membranes were first blotted with anti-phosphotyrosine antibody and later stripped and blotted again with anti-Dap12 antibody and anti-actin. Panel c: After AL002a stimulation, WT or TREM2-/- BMM were lysed and immunoprecipitated with Syk antibody. The membranes were first blotted with anti-phosphotyrosine antibody and later stripped and blotted again with anti-Syk antibody