Fig. 3
Deletion of myeloid Panx1 improves behavioral outcomes after TBI. a The rotarod test was used to evaluate latency to fall in sham Panx1fl/fl (grey open circles) and Cx3cr1-Cre::Panx1fl/fl mice (black open circles), as well as CCI Panx1fl/fl (orange closed circles) and Cx3cr1-Cre::Panx1fl/fl mice (blue closed circles). The test was performed 1-, 3-, and 5-days post-injury. Values are represented as mean ± SD (n = 8 per group). Linear regression analysis was used to determine the statistical difference between different groups at each time point (P values calculated for treatment and treatment*time). ***P < 0.0001, sham Panx1fl/fl vs CCI Panx1fl/fl; ###P < 0.0001, ##P < 0.001, sham Cx3cr1-Cre::Panx1fl/fl vs. CCI Cx3cr1-Cre::Panx1fl/fl. b Mice were tested in Barnes maze 7 days after sham or CCI. The average number of errors per day during the 4-day acquisition period was measured in sham Panx1fl/fl (grey open circles) and Cx3cr1-Cre::Panx1fl/fl mice (black open circle), as well as CCI Panx1fl/fl (orange closed circle) and Cx3cr1-Cre::Panx1fl/fl mice (blue closed circles). Values are represented as mean ± SD (n = 4 to 8 per group). Linear regression analysis was used to determine the statistical difference between different groups at each time point (P values calculated for treatment and treatment*time). ***P < 0.0001, sham Panx1fl/fl vs CCI Cx3cr1-Cre::Panx1fl/fl; ###P < 0.0001, sham Panx1fl/fl day 1 vs sham Panx1fl/fl day 3 and day 4. c Representative image of mouse track during the probe trial (“T” represents target quadrant). d Bar charts of the proportion of time spent in the target quadrant during the probe trial (*P < 0.05; 2-way ANOVA followed by Tukey’s multiple comparisons test)