Fig. 3

Effects of rhFGF21 on microglial polarization. a Representative pseudocolor and histograms of flow cytometry show the gating strategy for microglia (CD11b⁺CD45^int) and CD68⁺, CD86⁺, and CD206⁺ expressing microglia in cell suspensions from ischemic hemispheres. All gates were set using FMO control samples. b Bar graph summarizing the cell counts of microglia (CD11b⁺CD45^int) and CD68⁺, CD86⁺, and CD206⁺ expressing microglia in the brain 3 days after MCAO. c–f Quantification of flow cytometry shows the number of microglia (c) and their expression of CD68 (d), CD86 (e), and CD206 (f) at 1, 3, and 7 days. g Sketch picture indicating the area of the immunofluorescence pictures obtained from the penumbra of the infarct cortex. h Immunofluorescence staining shows CD16/32 expression by microglia (Iba-1) in the peri-infarct area at 3 days after MCAO under confocal observation. i Quantification of the counts of CD16/32⁺ microglia. n = 6 in sham the group, n = 12 in the MCAO and rhFGF21-treated group. Values are mean ± SEM, p value (red) of MCAO versus sham, p value (green) of MCAO versus MCAO+rhFGF21