Fig. 8

Effect of rhFGF21 on the inflammatory response in primary microglia in vitro. a, b Primary cultured microglia were exposed to OGD (a) or LPS (b) plus vehicle or rhFGF21 and subsequently subjected to qRT-PCR to detect the gene expression of pro-inflammatory molecules iNOS, CD86, TNF-α, IL-1β, and IL-6 and anti-inflammatory molecules CD206, Arg-1, IL-10, and IGF-1. c, d Dot plots (c) and summarized graph (d) based on the flow cytometry results show the expression of CD86 in primary microglia evoked by LPS. e Colocalization of NF-κB (green) with nuclei (blue) in Iba1 (red)-marked microglia revealed that the nuclear translocation of NF-κB was blocked by rhFGF21 but that the influence of rhFGF21 was reversed by PD173074. f Statistical analysis shows the counts of microglia in which NF-κB translocate into the nuclei. n = 4 per group. Values are mean ± SEM by one-way ANOVA