Fig. 5

BaP exposure exacerbated Aβ burden and plaque formation in APP/PS1 mice. a Representative images of immunohistochemical staining of human Aβ using 6E10 antibody in the cortex and the hippocampus of APP/PS1 mice. Total number of Aβ plaques was counted. b Thioflavin-T staining showed Aβ plaques in the cortex and the hippocampus of APP/PS1 mice. Number of Aβ plaques was counted. c Levels of RIPA soluble Aβ species and APP were detected by Western blot using 6E10 antibody for human Aβ/APP. β-actin was used to monitor loading errors. The ratio of densitometry values of Aβ monomer (measured in the image with long exposure time), oligomers, and APP (measured in the image with short exposure time) was normalized to β-actin. Data are expressed as a percentage of APP/PS1-Vehicle control and are mean ± SEM of 3–4 mice in each treatment group. Significance was determined by t test. **P < 0.01 compared with APP/PS1-Vehicle. Arrow indicates Aβ fibrils stuck in the loading well of the SDS-PAGE gel and then transferred to the PVDF membrane