Fig. 3
Activation of caspase-1 (a) Treatment of BLECs monolayers with PX (600 μM, 24 h) increased the activation of caspase-1, as assessed using fluorescent FLICACasp1 probes (methods). N = 14-15 from three independent experiments. (b) Representative images showing increased caspase-1 activation in BLECs monolayers treated with PX (600 μM, 24 h) ± VX-765 (50 μM). Scale bar = 200 μm (c) mRNA levels of caspase-1 was determined after 24 h treatment with PX, using RT-PCR. N = 3 biological repeats with 3 technical repeats. Data are normalized to control and presented as means ± SEM. *p < 0.05, ***p < 0.001 vs. control and ###p < 0.001 vs. PX